Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of Skp2 protein by siRNA in PFC in vitro by immunoflurescence. Immunoflurescence staining indicated high constitutive levels of Skp2 protein (arrow indication) in the nucleolus of PFC and NFC transfected with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). Transfection with Skp2 siRNA dramatically decreased the expression of Skp2 protein in PFC cells ( C , F ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, Staining, Transfection, Expressing

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of Skp2 protein by siRNA in PFC in vivo by immunoflurescence. Immunoflurescence staining indicated high constitutive levels of Skp2 protein in the nucleolus of PFC and NFC transfected with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). Transfection with Skp2 siRNA dramatically decreased the expression of Skp2 protein in PFC cells ( C , F ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, Staining, Transfection, Expressing

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA induced p27 kip1 accumulationin in PFC in vitro by immunofluorescence. Immunofluorescence staining indicated the expression of p27 kip1 dramatically increased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with PFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA induced p27 kip1 accumulationin in PFC in vivo by immunofluorescence. Immunofluorescence staining indicated the expression of p27 kip1 dramatically increased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with PFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Cell viability assay by MTT. After 6 to 12 days transfection with Skp2 siRNA, cell viability was significantly decreased in cultured hPFC cells compared with vehicle control and blank control epsecially on the 6th day after transfection. *p<0.01 versus vehicle and blank control; **p<0.05 versus vehicle and blank control.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: Viability Assay, Transfection, Cell Culture, Control

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA inhibited the cell proliferation of PFC in vitro (Brdu). For Brdu incorporation, cells growing on coverslips were incubated with Brdu. Incorporated Brdu was detected with antibodies as described in the Methods. Statistical analysis after cell counting showed that Brdu positive cells decreased after hPFC transfection with Skp2 siRNA compared with control cells. **p<0.01 versus vehicle and blank control.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, BrdU Incorporation Assay, Incubation, Cell Counting, Transfection, Control

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA inhibited the cell proliferation of PFC in vivo (Brdu). For Brdu incorporation, ptergium tissue were incubated with Brdu. Incorporated Brdu was detected with antibodies as described in the Methods. Statistical analysis after cell counting showed that Brdu positive cells decreased after hPFC transfection with Skp2 siRNA compared with control cells. **p<0.01 versus vehicle and blank control.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, BrdU Incorporation Assay, Incubation, Cell Counting, Transfection, Control

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of PCNA protein by siRNA in PFC in vitro and in vivo. Immunofluorescence staining indicated the expression of PCNA decreased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with hPFC and hNFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, In Vivo, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of PCNA protein by siRNA in PFC in vitro and in vivo. Immunofluorescence staining indicated the expression of PCNA decreased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with hPFC and hNFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, In Vivo, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA inhibited the proliferation of PFC and NFC in vivo. Hematoxylin and eosin staining was performed to examine the histological changes 14 days after transfection. Obvious PFC and NFC proliferation was detected in pSuppressor vehicle group or without transfection ( A , D , B , E ). There was little PFC and NFC proliferation in Skp2 siRNA transfection group ( C , F ). Scale bar is equal to 20 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, Staining, Transfection

Journal: World Journal of Surgical Oncology

Article Title: eIF6: a promising therapeutic target for gastric carcinoma via PI3K/AKT pathway modulation

doi: 10.1186/s12957-025-03746-w

Figure Lengend Snippet: Antibody

Article Snippet: CDK2 , Boster , PB9534 , 30 kDa , Rabbit.

Techniques:

Journal: Poultry science

Article Title: Dietary naringin supplementation on hepatic yolk precursors formation and antioxidant capacity of Three-Yellow breeder hens during the late laying period.

doi: 10.1016/j.psj.2023.102605

Figure Lengend Snippet: Figure 1. Appearance, index, and cell proliferation of the liver. a−cMean values with different superscripts letters denote statistical significantly difference (P < 0.05). Abbreviations: C, basal diet group; N1, N2, and N3, basal diet supplemented with 0.1%, 0.2%, and 0.4% of naringin, respec- tively. (A) Appearance of liver in each group. (B) Liver index (liver weight/body weight) in each group (n = 12). (C) Western blot analysis of CDK2 and PCNA in liver.TagedEnd

Article Snippet: Primary antibodies b-actin (1:1,000; BM3873), PCNA (1:1,000; BM0104), and CDK2 (1:1,000; PB0562), and secondary horseradish peroxidase-conjugated goat antirabbit (1:5,000; BA1054) and goat antimouse (1:5,000; BA1050) antibodies were purchased from Boster Biological Technology Co. Ltd. (Wuhan, China).

Techniques: Western Blot

Journal: The Journal of Biological Chemistry

Article Title: FAM129B/MINERVA, a Novel Adherens Junction-associated Protein, Suppresses Apoptosis in HeLa Cells *

doi: 10.1074/jbc.M110.175273

Figure Lengend Snippet: Intracellular localization of FAM129B in exponential and confluent cell cultures. A, HeLa cells (5 × 106) were harvested during the late exponential growth phase, and the cell extracts were fractionated into cytoplasmic (Cyt) and nuclear (Nuc) fractions (as described under “Experimental Procedures”). The fractions were analyzed by immunoblotting using antibodies directed against FAM129B, PARP, p53, CDK6, and CDK2. B, the same procedure was used to analyze NIH3T3 cell fractions. C, immunofluorescence microscopy of exponentially growing HeLa cells stained with a Hoechst 33342 DNA stain and with antibodies directed against rabbit FAM129B and the secondary antibody, chicken anti-rabbit IgG Alexa Fluor 594. The same protocol was followed for the cells in confluent HeLa cells (D) and an exponentially growing HeLa culture showing some mitotic cells (E). F, immunofluorescence co-localization (as described under “Experimental Procedures”) of FAM129B and β-catenin. The confluent cells were co-stained with rabbit antibodies directed against FAM129B and mouse antibodies directed against β-catenin. The secondary antibodies were Alexa Fluor 594-conjugated anti-rabbit IgG and an Alexa Fluor 488-conjugated anti-mouse IgG antibody. The cells were also stained with Hoechst 33342.

Article Snippet: Antibodies and Reagents Antibodies used for this study were rabbit anti-FAM129B (catalog no. 5122), rabbit anti-caspase-3 (catalog no. 9662), rabbit anti-cleaved caspase-3 (catalog no. 9664), rabbit anti-poly(ADP-ribose) polymerase (PARP 3 ; catalog no. 9542), rabbit anti-caspase-9 antibody (catalog no. 9502), mouse monoclonal anti-cdk6 (catalog no. 3136), mouse anti-caspase-8 (catalog no. 9746) (Cell Signaling, Beverly, MA); rabbit anti-cdk2 (catalog no. 21111) and rabbit anti-Akt (catalog no. 21054) (Signalway Antibody, Pearland, TX), mouse monoclonal antibody anti-β-catenin (catalog no. 610154) (BD Transduction Laboratories), mouse monoclonal anti-p53 (catalog no. sc-126), mouse monoclonal anti-GFP (catalog no. sc-9996), and mouse monoclonal β-tubulin (catalog no. sc-5274) (Santa Cruz Biotechnology, Inc., Santa Cruz).

Techniques: Western Blot, Immunofluorescence, Microscopy, Staining